| 초록 |
In order to targeting deliver drug to cancer cells, in this study, the surface of MNPs were co-decorated with epidermis growth factor fragment (EGFfr) instead of whole EGF because EGFfr is capable of binding to EGF receptors with comparable efficiency around 80%. Doxorubicin (DOX) was subsequently conjugated to the surface of MNPs through a pH sensitive hydrazone bond. In order to evaluate targeting effect of MNPs with EGFfr decoration rates of 10% and 40%, we prepared two EGFfr/DOX@MNPs with EGFfr to DOX conjugation ratio of 1 to 9 and 4 to 6. We also confirmed the release of DOX from DOX@MNPs at low pH, where DOX showed more rapid release compared with in physiological condition. In vitro cell uptake was also evaluated. EGFfr1/DOX9@MNPs showed much higher intracellular uptake in A549 cells than in CHO cells and also, cytotoxicity of EGFfr1/DOX9@MNPs was enhanced against EGF receptor overexpressed A549 cells because of EGFfr mediated cellular uptake of particles. |
