| 학회 |
한국공업화학회 |
| 학술대회 |
2008년 봄 (05/09 ~ 05/10, 한양대학교(안산)) |
| 권호 |
12권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Preparation of magnetic silica nanoparticles with metal affinity ligands for enzyme immobilization |
| 초록 |
Magnetic (Fe3O4) silica nanoparticles with immobilized metal affinity ligands were prepared for enzyme adsorption. Magnetite nanopaticles were synthesized by co-precipitating Fe2 and Fe3 in an ammonia solution. Then silica was coated on the Fe3O4 nanoparticles using a sol-gel method to obtain magnetite silica nanoparticles. The condensation product of 3-Glycidoxypropyltrimethoxysilane (GLYMO) and iminodiacetic acid (IDA) was immobilized on them and after charged with Cu2 , the magnetic silica nanoparticles with immobilizes were applied for the adsorption of His-tagged lipase. |
| 저자 |
김혜주, 황길수, 권혁성, 안익성
|
| 소속 |
연세대 |
| 키워드 |
magnetic nanoparticles; silica coating; metal affinity; enzyme aimmobilization; histidine-tag
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| E-Mail |
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