| 학회 | 한국공업화학회 |
| 학술대회 | 2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 | 24권 1호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | Mass production of scaffold DNA |
| 초록 | M13 phage is a filamentous phage that infects E. coli through F pilus. It is mainly used in phage display to find new antibodies. Also, its single strand circular DNA is used in DNA nanotechnology as a building block. We engineered 5’ UTR of protein V in M13 phage and screened 4 most efficient mutants. Production of M13 phage increased to 2.5 to 4.5-fold, and ssDNA production increased in 3.5 to 4.5-fold. Furthermore, we measured the engineered spacer expression levels by fusion of protein V with GFP. The fluorescence of the good producers is lower than the bad producers and the wild type. Also, the artificial expression of P5 using an inducible promoter shows that the concentration of P5 is inversely proportional to M13 phage and ssDNA production. |
| 저자 | 구본철, 이보영, 오민규 |
| 소속 | 고려대 |
| 키워드 | DNA nanotechnology; M13 phage; single strand DNA |
