| 학회 |
한국고분자학회 |
| 학술대회 |
2013년 봄 (04/11 ~ 04/12, 대전컨벤션센터) |
| 권호 |
38권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Preparation of polyplex formation via 3D microfluidic channel and its application to gene delivery system |
| 초록 |
Intracellular delivery of DNA is required to combine with a carrier system for efficient non-viral gene delivery. Negatively charged DNA can interact with positively charged polymer via electrostatic interaction and form polyplexes. These polyplexes are easy to prepare and efficiently translocate the genes into the cells. However, typical method of preparing polyplex has limitations in inhomogeneous particle size and surface charge density. Inhomogeneous particle size and surface charge density usually led to low transfection efficiency of polyplexes. Therefore, it is important to develop a novel method that preparing well defined polyplex particles. Here, we describe the new approach of preparing polyplexes using 3D microfluidic channel and application to gene transfection study. PAMAM dendrimer was used as cationic polymer and fabricated polyplex particles with pDNA using 3D microreactor and compared its physicochemical properties and transfection ability to bulk mixing method. |
| 저자 |
유광식1, 민경익2, 김동표2, 최준식1
|
| 소속 |
1충남대, 2POSTECH |
| 키워드 |
Gene delivery; Microfluidics; Polyplex
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| E-Mail |
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