| 초록 |
Over the past decade, intracellular gene delivery researches have been conducted effective methods for transfer therapeutic genes into cells. Polyamidoamine(PAMAM) dendrimer is widely using cationic polymer in the gene delivery system research. It has specially characteristics such as equal size distribution and low cytotoxicity and relatively good transfection efficiency but it indicates less transfection capacity in comparison with viral vector or Polyethyleneimine(PEI). Nuclear Localization Signal(NLS) Peptide is specific signal facilitating translocation into the nuclear and it enables inflow of optimum particle of 26nm whereas particles below 9nm can pass Nuclear Pore Complex(NPC) under absence of NLS peptide. The utilized NLS is KKKR peptide sequence of simian virus 40 T-antigen and it has rich primary amine group. In light of these information, The NLS peptide KKKR anticipated that it has possibility of proton buffering effect occurrence. |
