| 초록 |
The enzyme carbonic anhydrase (CA) catalyzes reversible hydration of CO2 and plays key physiological roles in various organisms. It has been recently suggested that this remarkably fast enzyme can be used for biomimetic CO2 sequestration, making this a promising alternative for chemical CO2 capture. In this talk, several strategies will be introduced for the development of efficient and cost-effective CO2-capturing nanobiocatalysts with genetically encoded functionalities. The strategies comprise i) engineering de novo disulfide bridges in recombinant CA enzyme, ii) construction of bacterial whole-cell catalyst with periplasmic CA, iii) auto-immobilization of CA in bioinspired silica nanoparticle by the silica-condensing peptide and iv) self-assembly of catalytic nanofilament by genetic fusion of CA to viral coat protein. Ongoing efforts will be also presented to make the CA-based biocatalysts better suited to practical applications. |
