| 초록 |
CH4 is considered as one of the most important greenhouse gases owing to its 20-fold higher heat capacity compared with that of CO2. Preliminary studies have aimed to elucidate the mechanisms of sMMO involved in this extremely stable C–H activation through intermediate studies, advanced spectroscopies, and structural researches, mostly in M. trichosporium OB3b and M. caps (Bath), although mechanistic studies are still required.M. sporium 5 was cultured in a regulated NMS media by supplying CH4 and air to understand its growth and the expression levels of multi-component enzymes. MMOH, MMOB and MMOR were found to be highly expressed in M. sporium 5, and it was purified to evaluate its catalytic activities using diverse substrates. The sMMO enzymes were utilized to measure SEA, and these results showed that M. sporium 5 exhibits optimal activity at pH 7.5. The electron transfer environment of MMOR is crucial for the activity of sMMO, and different acidities may change the electron transfer environment. In vitro activity measurements demonstrated that alkanes, halogens, benzene, and toluene are oxidized through sMMO, and 2 mol equivalents of MMOB showed optimal activity. |
