| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
Facile method for immobilization of genome editing protein based on dopamine polymerization |
| 초록 |
The CRISPR/Cas9 has attracted great attention due to the high simplicity and selectivity in editing the gene of interest. Immobilization of the CRISPR system on nonfunctionalized substrate surfaces with high efficiency would have various applications in biomedical research and diagnostic purposes. In this study, we introduce a facile method for surface immobilization of the CRISPR ribonucleoprotein mediated by polydopamine formation. A polypropylene surface was modified by dopamine polymerization, which can then readily react with the Cas9 protein by nucleophilic addition reactions. The efficiency of immobilization and bioactivity of Cas9 was characterized. The current approach can be used for immobilization of various proteins onto surfaces that are difficult to chemically functionalize while maintaining protein bioactivity. |
| 저자 |
이주훈, 정현정
|
| 소속 |
한국과학기술원 |
| 키워드 |
dopamine; CRISPR; point of care
|
| E-Mail |
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