| 초록 |
This study reports that a gallol moiety, the ubiquitous phenol unit found in plants, can be a functional red-color developing fluorogen. The newly synthesized fluorogen called GA-Rho can be easily produced by the condensation reaction between Gallol-containing Aldehyde with Rhodanine. With the previously presented scaffold protein, yellow fluorescence-activating and absorption-shifting tag (Y-FAST), the GA-Rho fluorogen bound to the protein exhibits red color fluorescent emission and reversibly turns on/off by PBS washing. Furthermore, we also demonstrated emission color interchanges of Y-FAST expressed in various cell lines via sequential treatment of spectral-tuned red, yellow, and green color fluorogens. This system can be widely applied as an adaptive reporter with dynamic temporal changes by multi-fluorescent emissions. Beyond the widespread uses of gallol derivatives for various fields, the gallol-mediated red color development opens a new direction in phenol-derivative research. |
