| 초록 |
To tune the detection range of a molecular beacon aptamer (MBA)/conjugated polyelectrolyte (CPE)-based ATP assay, electrostatic interaction between MBA and CPE was controled. Three kinds of CPEs (MP2 ~ MP6) were synthesized by changing the number of cationic groups (2 to 6) per repeat unit. In the absence of a target, open chain MBA enables the facile FRET from the CPEs to a fluorophore. By forming a G-quadruplex with a target, quenched PL signal was observed due to close contact between fluorophore and quencher. By increasing the ionic density of CPEs, confomational change toward open-chain MBA/CPE complexes was accelerated because of enhanced electrostatic, resulting in a detection range shift to higher [ATP]. By simply modifying the number of ionic groups in CPE, the population shift toward open-chain MBA/CPE demonstrates the successful tuning of detection range from nanomolar up to millimolar concentrations of ATP. |
