| 초록 |
Peptides have inherent characteristics which can cause various biological effect, therefore, they have been used in many biomedical fields including gene delivery systems. Here, we synthesized multifunctional peptide dimer (MPD) for gene delivery systems. The peptide monomer consists of cellular penetrating peptide moiety (R8), MMP-2 specific sequence (GPLGV), pH-responsive moiety (H5) and hydrophobic moiety (palmitic acid) (CR8GPLGVH5-Pal). MPD was formed by DMSO oxidation and MPD could condense pDNA successfully at a weight ratio of 2. MPD itself could self-assemble into sub-micron particles via hydrophobic interaction. MPD showed low cytotoxicity in comparison with PEI25k. MPD polyplex showed low transfection efficiency in HEK293 cells expressing no MMP-2 but high transfection efficiency in A549 cells expressing MMP-2, meaning the enhanced transfection efficiency by MMP-induced structural change. This result shows the potential of MPD for targeted gene delivery systems. |
