| 초록 |
The mortality rate for pancreatic cancer is high due to lack of powerful early diagnosis technologies. Main obstacles for improving clinical trial result are a diagnosis at an early stage and limited therapeutic efficacy. To overcoming this problem, recently tumor-derived exosomes are attracting interest in cancer diagnosis and treatment field. Here we demonstrate recent technologies highlighting the cancer exosome detection and isolation simultaneously by introducing lectin and Janus nanoparticles. Therefore, in this study, lectin proteins binding to glycans on the surface of pancreatic cancer cells were screened to develop exosome capturing technology based on lectin-sugar binding capacity instead of antibodies. The lectin-immobilized Janus nanoparticles were prepared and reacted with the target exosomes either in the tube or microchannel, confirming that the exosomes were successfully attached to the nanoparticles through fluorescent labels. |
