| 초록 |
Dietary polyphenols are important nutrients from foods which possesses complementary and alternative medicinal effects. The polyphenols are bulky biomolecules having structures like L-3,4-dihydroxyphenylalanine (DOPA), and the multiple hydroxyl groups are useful for adsorbing proteins. Herein, we have developed chemical modification and its stabilization method for preparation of HA-polyphenol derivatives. Four different polyphenols were successfully attached to hyaluronic acid (HA) using epichlorohydrin chemistry. 1H NMR and FTIR confirm the covalent bonding, whereas UV assay quantify its substitutions. The DPPH scavenging assay confirmed the recovered antioxidant scavenging activities even after chemical modification. Adhesion strength of HA derivative along the uniaxial tensile strain mode is almost double than the neat HA. This improved adhesion and antioxidant behavior of these derivatives is because of the introduced DOPA structure and multiple hydroxyl groups through polyphenols. |
