| 초록 |
A peptide with the sequence of RGD has been widely exploited to modify synthetic extracellular matrices, as it is known to bind specifically to integrin receptors of cells. In our previous study, adhesion and proliferation of fibroblasts cultured on alginate gels (2D system) were dramatically enhanced by chemical conjugation of RGD peptides to the alginate backbone, compared with unmodified alginate gels. However, cellular behaviour cultured within a three dimensional (3D) structure of polymeric scaffolds is often very different from two dimensional type culture. Our recent finding in 3D culture systems revealed that viability of fibroblasts within RGD-modified alginate hydrogels was much more improved than that within unmodified alginate gels. However, the total number of cells decreased over time, and the growth rate significantly decreased when cells were cultured within unmodified alginate gels, likely due to the lack of specific ligand-receptor interactions. |
