| 학회 |
한국화학공학회 |
| 학술대회 |
2004년 가을 (10/29 ~ 10/30, 호서대학교(아산캠퍼스)) |
| 권호 |
10권 2호, p.1550 |
| 발표분야 |
생물화공 |
| 제목 |
Regulatory metabolic flux analysis on different pH and temperature of Escherichia coli W3110 |
| 초록 |
Flux balance analysis (FBA) is a tool to estimate the intracellular flux on linear programming. In silico prediction of carbon source consumed and by-product excreted with the specific rate calculated by FBA of fermentation results of Escherichia coli W3110 supports the intracellular flux distributions on metabolic pathway are reliable. It should be not overlooked that FBA may lead the incorrect prediction when effects of regulation are not ignored. Regulations of Escherichia coli W3110 under different pH and temperature conditions from literature studies were applied to conventional metabolic flux analysis. Those applications of regulations based on the literature to do FBA were followed under different stress responses. Consequently, one can expect the rational intracellular flux of cell inside on the different pH and temperature experiments. Acknowledgement This work was financially supported by the Korean Systems Biology Research program (M10309090000-03B5002-00000) of the Korean Ministry of Science and Technology (MOST), the Brain Korea 21 of the Ministry of Education and LG chemicals Chair Professorship. Hardware for computational analysis was supported by the IBM-SUR program. |
| 저자 |
우한민, 김태용, 송효학, 이동엽, 이상엽
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| 소속 |
한국과학기술원 |
| 키워드 |
metabolic flux analysis; pH; temperature; regulation
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| E-Mail |
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| 원문파일 |
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