| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.448 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Neuronal differentiation of adult rat bone marrow derived stem cells on PLGA/SIS scaffold |
| 초록 | Bone marrow stromal cells (BMSCs) have pluripotent differentiation potential including differentiation into osteblasts, chondrocytes, adipocytes, and muscle cells by controlling cytokines in culture medium.1 BMSC activate endogenous restorative responses in in injured brain, which include angiogenesis, neurogenesis, and synaptogenesis.2 Meanwhile small intestimal submucosa(SIS) is a naturally occurring tissue matrix composed of extracellular matrix proteins and various growth factor. SIS is derived from the porcine jejunum and functions as a remodeling scaffold for a tissue repair.3 In this study, we reported that adult rat bone marrow stem cells can be induced to differentiate into neurons, potentially their usefulness in the treatment of neuronal disease. Different contents of SIS powder were included in each scaffolds; 0, 10, 20, and 50% of PLGA weight. We evaluated the scaffolds that were treated growth medium and neurogenesis medium after at 5 hours, 1, 3, 7, and 14 days. Degree of neurogenesis was evaluated by RT-PCR, immunohistochemical staining for the neuronal marker NSE and NF. The results of these experiments indicated that neuronal differentiation was occured well in the scaffold included 10% SIS. Acknowlegement This work was supported by KMOICE (N11-A08-1402-05-1-3). Reference 1. F. Sugiura, H. Kitoh and N. Ishiguro, B. B. R. C., 316, 233 (2004). 2. Michael Chopp and Yi Li. Neurology, 1, 92 (2002). 3. A. M. Woodsa, E. J. Rodenberg and M. C. Hiles, Biomaterials, 25, 515 (2004). |
| 저자 | 김순희1, 홍금덕1, 김선경1, 김문석2, 조선행2, 이종문1, 강길선1, 이해방2 |
| 소속 | 1전북대, 2한국화학(연) |
| 키워드 | Neurogenesis; SIS/PLGA scaffold |
