| 초록 |
The response of Mannheimia succiniciproducens to succinic acid shock was investigated based on global gene expression profiling. Microarray analysis revealed that cells elicited both general and specific transcriptome responses when challenged with 30 g/l succinic acid conditions over a 30-min period. Specific response to succinic acid stress included the up-regulation of genes encoding transporters (symporters, antiporters, diphosphate bond hydrolysis driven transporters, multi-drug exporter, dicarboxylate transporters), the lysR-type transcriptional regulator, and cell membrane structure. These results suggest that M. succiniciproducens regulates multiple transporter, cell envelope, and central intermediary metabolism as part of its transcriptome response to succinic acid shock.[This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (2005-01294). Further supports by the LG Chem Chair Professorship, IBM SUR program, Microsoft, and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated.]. |
