| 초록 |
In this study, we investigated the antibacterial properties of low molecular weight water-soluble chitosan (LMWSC; MW1, MW3, MW5 and MW10) using a combination of approaches, including MIC estimations at 2 different pH values, bactericidal kinetics, calcein-loaded liposomes, depolarization assay, and electron microscopy, as well as an in vivo study utilizing a wound infection and wound healing model. The degree of depolarization (in Escherichia coli, pH 7.4) and calcein leakage (lipid composition; PE/PG=7/3 (w/w), pH 5.4 and 7.4) were evaluated. Moreover, cells cultured at various pH values were evaluated by confocal microscopy (pH 5.4 and 7.4). The results revealed that treatment with LMWSC of different molecular weights had different effects on bacteria. This study was supported by IPET(Korea Institute of planning & Evaluation for Technology in Food, Agriculture, Forestry & Fisheries) Research Fund in 2009. |
