| 초록 |
The green alga, Chlamydomonas reinhardtii, is capable of sustained H2 photoproduction when grown under sulfur deprived conditions. We investigated continuous hydrogen production by sulfate readdition under sulfur deprivation. Readdition of small quantities of sulfate (0~120μM MgSO4) to cell suspensions results in an initial increase in culture density, an increase in rate of sulfate consumption, and an increase in the total amount of H2 produced, up to an optimal concentration (~30 μM MgSO4). However, the addition of too much sulfur (above 60 μM) was delayed the on set of hydrogen production and lowered the final yield of hydrogen production. Using these results, we attempted the continuous and sustained hydrogen production by cycling a single C. reinhardtii culture for up to 4 cycles. |
