| 학회 |
한국공업화학회 |
| 학술대회 |
2013년 봄 (05/01 ~ 05/03, ICC JEJU) |
| 권호 |
17권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Utrasensitive assay based on gold nanoparticle probes by aptamer PCR method for the detection of pathogen |
| 초록 |
The aim of this study is to develop an ultrasensitive detection system based on aptamer PCR technology using particle probes to apply to protein and pathogen. Single strand DNA bound on gold nanoparticle is used as a template for PCR. As this aptamer binds to an antibody, it is used to immobilize antibody on the gold nanoparticle probes. The bonding is carried out without complex conjugation chemistry. Rapid, simple and sensitive improvement is provided by the antibody-specific aptamer. Acknowledgments : This research was supported by the Nuclear R&D program through the National Research Foundation funded by the Ministry of Education, Science and Technology, Korea and by No. 20114010203090 and "Development of Sustainable Remediation Technology for Marine Contaminated Sediments" funded by the Ministry of Land, Transport and Maritime Affairs, Korea. |
| 저자 |
주정옥1, 김현수2, 최정우3, 오병근3
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| 소속 |
1Interdisciplinary Program of Integrated Biotechnology Sogang Univ., 2Department of Chemical & Biomolecular Engineering Sogang Univ., 3Department of Chemical & Biomolecular Engineering Sogang Univ. / Interdisciplinary Program of Integrated Biotechnology Sogang Univ. |
| 키워드 |
gold nano particle probes; E.coli O157:H7
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| E-Mail |
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