| 학회 |
한국고분자학회 |
| 학술대회 |
2013년 가을 (10/11 ~ 10/12, 창원컨벤션센터) |
| 권호 |
38권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Gene delivery to CD44-positive cancer cells using Hyaluronic acid-ceramide (HA-CE) based Liposomes |
| 초록 |
Hyaluronic acid-ceramide (HA-CE) modified liposomes were designed using DOTAP/DOPE for targeted delivery of therapeutic genes to the CD44-overexpressing cancer cells. The size and zeta potential of HA-CE based liposomes was characterized by DLS and found 270±10 nm and +30 mV respectively. Then lipoplex prepared at different N/P ratios showed strong DNA binding affinity. Cytotoxicity was evaluated by WST assay for different cell lines such as MDA MB231, MCF7 and NIH3T3 cells. In case of NIH3T3 cell, liposome showed negligible cytotoxicity. However CD44 positive cells; MDA MB231 and MCF7 exhibited little cytotoxic effect because of higher targeting for HA-CE based liposome. Then we have examined the transfection efficiency of liposome using luciferase reporter plasmid DNA. We found transfection efficiency of HA-CE based liposome was remarkably higher in case of MDA MB231 cells. |
| 저자 |
수딥타 말릭, 최준식
|
| 소속 |
충남대 |
| 키워드 |
Gene delivery; CD44 receptor
|
| E-Mail |
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