| 초록 |
Polyphenols are widely distributed in vegetable, herbs, fruits and beverages and have attracted attention in health benefits including antioxidants, anti-ageing etc. The polyphenols are biomolecules comprising metal binding sites like catechol in L-3,4-dihydroxyphenylalanine (DOPA), and adjacent hydroxyl-keto functionalities. In this work, we have chemically attached polyphenols to hyaluronic acid (HA) using epichlorohydrin chemistry and final HA conjugated polyphenols were cross-linked with metal binding sites. The 1H NMR and FT-IR confirmed the covalent bonding, whereas UV assay quantified its substitution. Adhesion strength of HA derivatives under the uniaxial tensile strain mode is more than double that of the neat HA. The metal ion binding of conjugated polymers have confirmed by monitoring its hydrogel formation in aqueous condition. This improved adhesion and hydrogel formation behavior of these derivatives possibly resulted from the introduced polyphenols of DOPA structure and metal binding functionality. |
