| 학회 |
한국화학공학회 |
| 학술대회 |
2003년 가을 (10/24 ~ 10/25, 한양대학교) |
| 권호 |
9권 2호, p.2137 |
| 발표분야 |
생물화공 |
| 제목 |
Characterization of ε-caprolactone production by recombinant Escherichia coli strain harboring cyclohexanone monooxygenase gene |
| 초록 |
Cyclohexanone monooxygenase (CHMO) catalyzing Baeyer-Villiger oxidation converts cyclic ketone compounds to optically pure lactone derivatives which are used as platform materials in chemical industry. A recombinant Escherichia coli system was designed to express CHMO from Acinetobacter sp. NCIB 9871 and to optimize microbial production of ε-caprolactone from cyclohexanone. Maximum specific CHMO activity of 290 U/g cellular protein was obtained in a batch fermentation at 25℃ and 0.01 mM isopropyl-thio-β-D-galactopyranoside induction. To overcome the inhibitory effects of cyclohexanone on cell growth and ε-caprolactone production, a fed-batch fermentation was performed with continuous feeding of cyclohexanone at a rate of 0.15 g/l-hr. Low level of cyclohexanone under 0.4 g/l increased ε-caprolactone concentration to 1.2 g/l and maintained its productivity at 0.08 g/l-hr. |
| 저자 |
이원흥, 김명동, 이대희, 서진호
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| 소속 |
서울대 |
| 키워드 |
cyclohexanone; cyclohexanone monooxygenase; ε-caprolactone; fed-batch fermentation; recombinant <i>Escherichia coli</i> |
| E-Mail |
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| 원문파일 |
초록 보기 |
