| 초록 |
Cationic antibacterial peptides are the most abundant antibiotics in nature and have been frequently proposed as new anti-infective agents. In this study, a set of diastereomeric peptides is researched about their antibiotic activity against multiple drug resistant clinical isolates and their modes of action against Gram-positive cocci. The tested diastereomers displayed high antimicrobial and broad spectrum activity with D-P5-18mer. The antimicrobial activity of diastereomeric P5-18mer was two times stronger against Gram-negative bacteria than either CA-MA-20mer or P5-18mer. When tested against ten clinically isolated antibiotic-resistant strains in the presence of 0, 150 or 300 mM NaCl, diastereomeric-P5-18mer retained strong activity against all bacteria, yet showed little or no cytotoxicity against the human keratinocyte cell line. Finally, D-P5-18mer showed resistance against trypsin digestion unlike other analogues. |
