| 학회 |
한국고분자학회 |
| 학술대회 |
2013년 봄 (04/11 ~ 04/12, 대전컨벤션센터) |
| 권호 |
38권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Cationic amino acid - conjugated polyamidoamine(PAMAM) dendrimer for enhanced transfection efficiency |
| 초록 |
Gene transfer using non-viral gene delivery carrier is being performed instead of viral gene delivery carrier that tend potent cytotoxicity. However non-viral gene delivery carrier has low transfection efficiency. We introduced Polyamidoamine (PAMAM) dendrimer(generation 4) that is widely used as a non-viral and cationic vector for gene delivery. Amino acid - conjugated PAMAM dendrimer induce not only enhancement of transfection efficiency but also a decrease in toxicity. We synthesized PAMAM G4-Histidine-Lysine(G4HK) / Ornithine(G4HO) and compared this study with previous results(PAMAM G4–Histidine-Arginine, G4HR). Cationic amino acid contributes to condensation of DNA and contact with cell surface. Histidine has Imidazole group that induce buffer(proton sponge) effect in endosome. |
| 저자 |
박정현, 최준식
|
| 소속 |
충남대 |
| 키워드 |
PAMAM; transfection; cationic amino acid
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| E-Mail |
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