| 초록 |
Bioreducible cationic polymers have been synthesized to deliver mRNA into the cells. mRNA is genetic material generated by transcription of DNA and these ssRNA molecules are employed to induce the protein expression of target genes. mRNA can be an alternative genetic material which can be expressed in mammalian cells instead of plasmid DNA (pDNA). It has multiple advantages over pDNA such as 1) fast on-set of target gene expression, 2) less genetic stress on host cells, 3) no nuclear localization is required. In this study, we have prepared GFP and Firefly Luciferase mRNA by in vitro transcription (IVT) method. Various bioreducible polymeric systems have been screened to optimize their mRNA delivery efficiency. Transfection and expression of target genes have been confirmed in Mouse embryonic fibroblast (MEF) cells. |
