| 초록 |
Development of a new substrate for surface-enhanced Raman scattering (SERS) is one area of interest for improvement of SERS performance. Herein, we describe a method to develop new mesoporous SERS substrates for sepsis detection using M13 phages that display cysteine-rich peptides on the pVIII major units. The substrates were characterized and magnetized with magnetic immuno colloids made of gold-coated magnetic nanoparticles and specific antibodies. The SERS-tags prepared by gold nanoparticles are modified with Raman dyes and antibodies are immobilized to capture the biomarkers in the serum samples. The SERS-tags are bound to the mesoporous substrate via antibody/antigen interactions to form clusters or layer-by-layer assemblies of SERS-tags for Raman signal enhancement. The SERS spectra show distinct peaks for tags corresponding to three typical sepsis-specific biomarkers for diagnostics with limit of detection of 27 pM, 103 pM, and 78 pM for CRP, PCT, and sTREM-1, respectively. |
