| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 봄 (04/08 ~ 04/09, 대전컨벤션센터) |
| 권호 |
35권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Detection ofprotease activity in 96-well plates using a polymer-conjugated fluorogenic probes |
| 초록 |
The extent of matrix metalloproteinases(MMPs) detected in biological samples provides important clinical information for diagnosis, prognosis, and therapeutic monitoring of various diseases relating with MMPs. We developed a new high-throughput MMP diagnostic kit (MMP-D-KIT) based on a 96-well plate by immobilizing MMP-13 specific fluorogenic peptide probes, which is a pair consisting of a near-infrared fluorophore and a quencher, on to glycol chitosan polymer anchored 96-will plate. When MMP enzymes were simply added in a MMP-D-KIT, the fluorescence of each well was recovered and the fluorescence intensity showed distinct difference within minutes through NIR fluorescence imaging system. Furthermore, recovery of NIR fluorescent signals in MMP-D-KIT was proportional to MMP’s concentration. Additionally, the MMP-D-KIT was used to detect MMP activity in synovial fluid from 12 OA patients. The fluorescence intensity measured using MMP-D-KIT decidedly correlate with the progression of OA. |
| 저자 |
류주희1, 이애주1, 박종웅2, 김광명1, 권익찬1, 최귀원1, 윤인찬1
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| 소속 |
1한국과학기술(연), 2고려대 |
| 키워드 |
matrix metalloproteinase; diagnostic kit; glycol chitosan
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| E-Mail |
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