| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 가을 (10/11 ~ 10/12, 일산킨텍스) |
| 권호 |
32권 2호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
A Novel Hyaluronic acid - Peptide Conjugate by Michael Addition for Formyl Peptide Receptor Like - 1 Receptor |
| 초록 |
Hyaluronic acid (HA) - peptide (WRYMVm) conjugate for formyl peptide receptor like - 1 (FPRL-1) receptor was successfully synthesized. Aminoethyl methacrylated HA (HA-AEMA) was synthesized and conjugated to C-WRYMVm via Michael addition reaction between metharylate groups and thiol groups in cysteine. HA-peptide conjugate was purified by fractionation using gel permeation chromatography (GPC). The formation of HA-peptide conjugate could be confirmed by GPC and 1H nuclear magnetic resonance (NMR) analysis. The peptide content in HA-peptide conjugate was quantified by measuring the absorbance at 280 nm with GPC. The biological activity of HA-peptide conjugate was confirmed by in vitro RBL-2H3 cell activity tests measuring the elevation level of phospho-extracellular signal-regulated kinase (pERK). The novel bioconjugation of HA-AEMA to peptide molecule with thiol groups might greatly contribute to elongate its half-life, which can be successfully applied for various biopharmaceuticals. |
| 저자 |
오은주, 한세광
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| 소속 |
포항공과대 |
| 키워드 |
Hyaluronic acid; Formyl peptide receptor like - 1; Conjugation; Michael addition; Phospho-extracellular signal-regulated kinase
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| E-Mail |
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