| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
(콜로이드계면화학)첨단 소재 및 바이오 기술 분야의 표면화학 연구동향 |
| 제목 |
Myristoyl switch modulating reversible protein translocation to lipid membranes and palmtylation of a protein for albumin binding |
| 초록 |
Fluorescence approaches have been widely used for elucidating the dynamics of protein-membrane interactions in cells and model systems. However, non-specific multi-site fluorescent labeling often results in a loss of native structure and function. Here, we develop a method using genetic incorporation of non-natural amino acids and bio-orthogonal chemistry to site-specifically label with a single fluorescent small molecule or protein the myristoyl-switch protein recoverin, which is involved in rhodopsin-mediated signaling in mammalian visual sensory neurons. We demonstrate reversible Ca2+-responsive translocation of labeled recoverin to membranes. Genetic incorporation of non-natural amino acids and bio-orthogonal chemistry were also used to conjugate human serum albumin (HSA) or HSA ligand to therpaeutic proteins for the prolonged serum half-life. |
| 저자 |
권인찬 |
| 소속 |
광주과학기술원 |
| 키워드 |
protein translocation; lipid membrane; albumin
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| E-Mail |
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