| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.682 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | The Chitosan Microsphere for a Nasal Vaccine Delivery System in vitro and in vivo |
| 초록 | Chitosan is a valuable excipient for nasal drug delivery system due to its biocompatibility, biodegradability and low cost. Also, it has been reported that chitosan could induce cytotoxic T lymphocytes and macrophage activation [1]. Poly(ethylene glycol) (PEG) is a water-soluble amphipathic polymer that exhibits properties such as protein resistance and low toxicity [2]. Chitosan microspheres (CMs) and PEGylated chitosan microspheres (PCMs) were prepared based on ionic gelation process of tripolyphosphate and then, the microspheres were loaded with Bordetella Bronchiseptica Dermonecrotoxin (BBD), a virulence factor of a causative agent of atrophic rhinitis, for nasal vaccination as shown in Fig. 1. Particle sizes of BBD-loaded CMs and BBD-loaded PCMs were smaller than 10 μm and had narrow size distribution with uniformity as shown in Fig. 2. Mechanical stability, vaccine BBD release from BBD-loaded microspheres and secretion of cytokine such as tumor necrosis factor-α (TNFα) and nitric oxide (NO) from BBD-loaded CMs and BBD-loaded PCMs were checked in vitro. Also, BBD-loaded CMs were treated to mouse as a model animal, and systemic immunity and nasal immunity were checked in vivo. [1] P. Artursson, T. Lindmark, S.S. Davis, L. Illum, Effect of chitosan on permeability of monolayers of intestinal epithelial cells (Caco-2), Pharm. Res. 11, 1358 (1994). [2] O. Tatsuro, N. Hidetoshi, O. Yuichi, Aggregation phenomenon of PEG-grafted chitosan in aqueous solution, Polymer 39, 5171 (1998). |
| 저자 | 강호림1, 박인규1, 강상균1, 유한상1, T. Akaike2, 조종수3 |
| 소속 | 1서울대, 2Tokyo Institute of Technology, 3Japan |
| 키워드 | chitosan; microsphere; nasal vaccination |
