| 초록 |
Glutaric acid is a promising C5 dicarboxylic acid having wide applications in the biochemical industry. Glutaric acid can be produced by fermentation and bioconversion. The purpose of this study is to enhance the stability and reusability of this whole-cell system for realizing the efficient production of glutaric acid under the harsh reaction conditions. Toward this end, various matrices were evaluated to entrap Escherichia coli whole-cell overexpressing 4-aminobutyrate aminotransferase (GabT), succinate semi-aldehyde dehydrogenase (GabD), and NAD(P)H oxidase (NOX). PVA-PEG gel was selected for cell entrapment, and several parameters of the reaction were optimized. Treatment of Tween 80 as a surfactant and additional NOX were found to be effective. Under the optimized conditions, most activity was retained even after the eighth cycle while free cells lost most of their activity after only two cycles. |
