초록 |
SiRNA hold great potential for tumor treatment. But, it is difficult to reach the target due to low stability. Even if penetration of siRNA into target cell, an endosomal escape must occur to have siRNA effect. Here, we developed liposomal siRNA delivery system labeled with EphB4 peptide to target EphB4 receptor on the surface of ovarian tumor cells. Additionally, we developed bubble generating thermosensitive liposomes (BTSL) that can be triggered release of drug by near infrared (NIR). Size of stat3 siRNA-BTSL and EphB4-stat3 siRNA-BTSL were 106.76 ± 3.85 nm and 110.87 ± 0.51 nm, and surface charge was -34.9 mV and -38.2 mV, respectively. Loading efficiency of siRNA was 93.2 %. Intracellular delivery of EphB4-stat3 siRNA-BTSL was highly increased EphB4 positive HeyA8 than EphB4 negative RMG2. Release of siRNA from BTSLs showed burst release by bubble generation into BTSLs stimulated with NIR. This study demonstrate that NIR triggered endosomal escape will more effective treatment. |