| 학회 |
한국화학공학회 |
| 학술대회 |
2011년 봄 (04/27 ~ 04/29, 창원컨벤션센터) |
| 권호 |
17권 1호, p.368 |
| 발표분야 |
생물화공 |
| 제목 |
Enhancement of mussel-originated silk-like protein expression using baculoviral polyhedron |
| 초록 |
Recombinant spider and silkworm have been studied as silk materials for several decades. Even though bees and ants have been focused as new silk-bearing organisms, the research for silk proteins from other organisms is rare. Especially, there is no precedent about silk-like proteins from marine organisms. Comparing with the silk genes from spider and silkworm, we found that marine mussel has silk-like gene that consists of many repeats with abundance of glycine and alanine. We designed mussel-derived silk gene which contains alternating repeated (hydrophobic) and non-repeated regions. Because we observed prominent retardation of cell growth by expression of mussel silk-like protein, fusion strategy with the baculoviral polyhedrin was used to induce insoluble expression of target silk protein. Finally, we could express the mussel silk-like proteins as insoluble form with high cell growth. |
| 저자 |
양윤정1, 최유성2, 차형준1
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| 소속 |
1Department of Chemical Engineering, 2Pohang Univ. of Science and Technology |
| 키워드 |
Mussel; Silk-like protein; Baculoviral polyhedrin
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| E-Mail |
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| 원문파일 |
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