| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 가을 (10/11 ~ 10/12, 일산킨텍스) |
| 권호 |
32권 2호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
In Vivo Cytotoxicity and Biodistribution of Cationic Emulsion-Mediated Gene Delivery Systems as Non-Viral Vectors |
| 초록 |
Non-viral gene delivery system is attractive due to low toxicity and reproductive transgene expression compared to viral vector commonly used in gene therapy protocols. We developed sufficiently stable and biocompatible emulsion complex, which was positively charged and spherical. This study evaluated the safety and the gene transfection ability of the complex in vitro and in vivo by compared with commercial cationic reagent (PEI, 25kDa) and liposome. We used a luciferase reporter gene to monitor the biodistribution properties of the emulsion vehicles and to assess the patterns of gene expression in real-time. We found that, while PEI and liposome showed relatively low level of gene expression, emulsion-based bioluminescence showed very stable and efficient gene expression profiles over 8 days. These data demonstrated the feasibility of lipid emulsion as in vivo gene carrier, which could be useful in optimizing the gene therapy approaches for treatment of liver-specific diseases. |
| 저자 |
남혜윤1, 김광명1, 권익찬1, 박재형2, 정서영3
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| 소속 |
1한국과학기술(연), 2경희대 환경응용화학대, 3경희대 나노의약생명과학과 |
| 키워드 |
non-viral vector; gene delivery; cationic lipid emulsion; in vivo imaging
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| E-Mail |
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