| 학회 |
한국고분자학회 |
| 학술대회 |
2015년 봄 (04/08 ~ 04/10, 대전컨벤션센터) |
| 권호 |
40권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 II |
| 제목 |
Facile spheroid forming method using thermo-sensitive hydrogel based on glycol chitosan |
| 초록 |
An in vitro cell culture process to obtain transplantable cells is required for tissue engineering or cell therapy. A two-dimensional (2-D) monolayer culture method has been a standard to obtain transplantable cells. However, in vivo system is 3-D and present differences from 2-D in protein expression, cell proliferation, extracellular matrix, and metabolic function. This may be one of the major reasons for limiting the therapeutic efficacy of transplanted cells. Thus one of the thrusts in the field of cell therapy is to develop better 3-D cell culture method in vitro that would mimic in vivo system. The purpose of this study was to investigate a facile, consistent, and cost-effective 3-D cell culture method in vitro. To that end, thermo-sensitive hydrogel that have reversible sol-gel transition was synthesized and was coated on cell culture plate for 3-D cell culture. This work was supported by Project No. PJ009956 |
| 저자 |
조명옥1, 심혜은2, Zhengzheng Li1, 김기석2, 허강무1, 강선웅2
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| 소속 |
1안전성평가(연), 2충남대 |
| 키워드 |
3-D culture; Spheroid; Chitosan; Thermo-sensitive hydrogel; Cardiomyocytes
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| E-Mail |
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