| 학회 |
한국공업화학회 |
| 학술대회 |
2004년 가을 (10/29 ~ 10/30, 호서대학교(아산캠퍼스)) |
| 권호 |
8권 2호 |
| 발표분야 |
생물공학 |
| 제목 |
Structure-switching Aptamer for detection of proteins |
| 초록 |
A new method of aptamer-protein binding has been developed using fusion aptamer containing no chemical modification. We prepared the fusion aptamer which connected two aptamers. The fusion aptamer consist of three parts. The first part is the malachite green (MG) aptamer as signaling domain, the second part is HCV replicase aptamer as recognition domain and the third part is inducing domain to induce a conformational change of the fusion aptamer. It transduce the recognition signal through allosteric regulation of interaction with MG and target protein. We tested the fusion aptamer for target protein, HCV replicase. We were able to detect the binding event by monitoring the fluorescence change of MG. The fluorescence of MG increased upon adding HCV replicase. |
| 저자 |
방경숙, 조수형, 김병기
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| 소속 |
서울대 |
| 키워드 |
Switching aptamer; Allosteric; Protein; Fluorescence
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| E-Mail |
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