| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
Multiplex Immunoassay Using Hydrogel Microparticles Functionalized After Fabrication |
| 초록 |
Protein detection and quantification are increasingly becoming important analytical practices in various applications from biopharmacy to medical diagnostics. With an increasing demand for simultaneous measurement of multiple analytes in a single sample, bead-based platforms have emerged at the forefront of multiplex technology. Hydrogel beads have been proposed to combine hydrogel’s solution-like reactivity and non-fouling characteristic. However, their synthesis is deemed unreliable and delicate because the photoinitiators used for radical polymerization of hydrogels are incompatible with probe antibodies. This causes the antibodies to aggregate and lose their affinity to their target protein. In this work, we introduce hydrogel microparticles that are functionalized after synthesis and demonstrate their advanced multiplex protein detection performance. Our technique eliminates the aggregation of antibodies and features a high sensitivity, a fast detection, and a broad assay range. |
| 저자 |
이현지1, 노윤호1, 김현웅1, 김선민2, 임용준1, 봉기완1
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| 소속 |
1고려대, 2보라매병원 |
| 키워드 |
Immunoassay; multiplex detection; hydrogels; microparticles; microfluidics
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| E-Mail |
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