초록 |
Sewage sludge after lipid extraction for biodiesel production was used to obtain sugars for bioethanol production. The extraction of sugars was carried out by dilute-acid pretreatment, followed by enzymatic saccharification. The optimal pretreatment conditions were optimized through response surface methodology, and these were 120 °C, 85 min and acid concentration of 5.9%(v/v). Under these conditions, sugar yield was 75.5%. Enzyme mixtures (celluclast: viscozyme) at the total volume and ratio of 1:2 showed the highest sugar yield (31.4%). Consequently, more than 90% of sugar was extracted from the sewage sludge. Finally, commercial yeasts from six manufacturers were evaluated for bioethanol production, and the optimum microorganism successfully produced bioethanol using the sugars from sewage sludge. |