| 초록 |
The anticancer activity of heparin due to the inhibition of angiogenesis has recently attracted considerable attention in pharmaceutical fields. Heparin, highly anionic polysaccharide, is applicable to protein carriers because it also has strong affinity with cationic proteins. In this study, our group developed a protein carrier retaining anticancer activity, a self-assembled nanogel of heparin-Pluronic (HP) conjugate. The synthesized HP conjugate was characterized. The bioactivity of the HP conjugate that roughly indicates the anticancer activity, of HP was measured to be about 15 and 20% APTT and Anti-FXa assay, respectively. The DNase-loaded HP nanogels prepared by direct dissolution method showed high loading efficiency, efficient size and round shape. In HeLa cell culture, HP nanogels exhibited low cytotoxicity and high intracellular uptake efficiency. Evaluating the anticancer activity and cytoplasmic release behavior of DNase-loaded HP nanogels is now in progress. |
