| 초록 |
Targeted and strong immune stimulation to antigens have been applied to cure immune-related diseases using adjuvants. CpG have been known as nucleic acid-based adjuvant to evoke the adaptive immune response by activating immune cells. To use CpG as an effective adjuvant, CpG should be delivered to immune cells specifically and stably. Here, we have synthesized a novel type of CpG embedded microparticle (CpG-MP) as an immunostimulant. DNA strands bearing CpG were elongated and self-assembled into homogeneous micron-sized CpG-MP via complementary rolling circle amplification. CpG-MP released CpGs in phagolysosome mimicking condition and kept its structure in serum condition. Macrophage could readily swallow CpG-MP through phagocytosis without additional carriers, followed by the expression of cytokines. Further in vivo test, CpG-MP-1.0 could arise OVA-specific immune response. Altogether, CpG-MP could be used as a stimulator for induction strong adaptive immune response. |
