| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 봄 (04/22 ~ 04/23, 대구 EXCO) |
| 권호 |
16권 1호, p.403 |
| 발표분야 |
생물화공 |
| 제목 |
Immobilization of cystein-tagged Protein G on amine modificated silica coated magnetic nanoparticles |
| 초록 |
This work reported the immobilization of cystein-tagged Protein G onto amino modifiedmagnetic nanoparticles(MNPs) for control and enhancement ofantibody orientation and immobilization. Portein G wasobtained from genetic engineeredStreptococcus aureus as an activated site for antibody and magnetic nanoparticles were used the 30nm of silica-coated iron oxides. The overal process involved the conjugation of amino-functionality of MNPsand thiol group of cross-linkerin N-terminal ofprotein G. The conjugation efficiency was calculated over 40%. The synethetic routes was confirmed by the detailed characterization such as ATR FT-IR,andDRIFT UV-Vis.
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| 저자 |
최홍경1, 안치영2, 김중배3, 이진형1, 장정호4
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| 소속 |
1Bio-IT Convergence Center, 2Korea Institute of Ceramic Engineering / Department of chemical & biological engineering, 3Korea Univ., 4Korea Institute of Ceramic Engineering |
| 키워드 |
Magnetic nanoparticle; silica magnetic nanoparticle; Protein G; immobilization
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| E-Mail |
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| 원문파일 |
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