| 학회 |
한국화학공학회 |
| 학술대회 |
2016년 가을 (10/19 ~ 10/21, 대전컨벤션센터) |
| 권호 |
22권 2호, p.1814 |
| 발표분야 |
생물화공 |
| 제목 |
Metabolic Engineering using Synthetic Small Regulatory RNA based Rapid Gene-Knockdown Technique for Enhanced Production of Cadaverine and Tyrosine |
| 초록 |
Small regulatory RNAs (sRNAs) are gene regulators that act on post-transcriptional phase. Here, we developed synthetic a sRNA gene knockdown technique. Synthetic sRNA properly repressed DsRed2 and was used to enhance the production of tyrosine and cadaverine. Through screening of 14 different strains with sRNAs, we isolated a strain producing 2 g per liter of tyrosine and using a library of 130 synthetic sRNAs, murE was isolated and showed a 55% increase in cadaverine production. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557); the Intelligent Synthetic Biology Center through the Global Frontier Project (2011-0031963) of the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea; the Commercializations Promotion Agency for R&D Outcomes(COMPA-2015K000365) funded by the Ministry of Science, ICT and Future Planning(MISP).] |
| 저자 |
Robert Binkley1, 노민호1, 나도균2, 유승민1, 이상엽1
|
| 소속 |
1KAIST, 2중앙대 |
| 키워드 |
대사공학 |
| E-Mail |
|
| 원문파일 |
초록 보기 |
