| 초록 |
The CRISPR system is a highly efficient tool for cellular reprogramming in cancer. Co-expression of Cas9 and sgRNA using plasmid has a good gene editing efficacy, but its transport into the nucleus remains a challenge due to its large size. Herein, we used polyethyleneimine carbon dots (PEI-Cdot) to package all-in-one plasmid encoding Cas9, GAL4UAS-luciferase sgRNA, and mCherry, and the complexes were coated with hyaluronic acid (HA) to target CD44 expressing tumors. The complexes were characterized by measuring hydrodynamic size, zeta potential and TEM. We established GAL4UAS-luciferase-expressing CD44 positive-HeLa cell line. By monitoring the expression of mCherry, we confirmed that the HA-functionalized PEI-Cdot complexes resulted in efficient delivery of the all-in-one plasmid. Finally, genome editing of HA-functionalized PEI-Cdot complexes delivered to GAL4UAS-luciferase-expressing HeLa cells was investigated by examining the knockout efficiency of the luciferase gene. |
