| 학회 | 한국고분자학회 |
| 학술대회 | 2003년 가을 (10/10 ~ 10/11, 부경대학교) |
| 권호 | 28권 2호, p.86 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Chondrogenesis of hMSC using thermosensitive chitosan for urinary application |
| 초록 | Previously, we have reported the synthesis and characterization of thermosensitive chitosan (chitosan-g-NIPAAM) as a injectable biomaterials. This materials has been prepared by grafting N-isopropylacrylamide)(NIPAAm) to chitosan bacbone using Ce(IV) initiator. These copolymers have demonstrated unique properties, when characterized by spectroscopy, thermal analysis, low critical solution temperature measurements. The aim of this study was to assess the ability to differentiate from mesenchymal stem cells (MSCs) to chondrocytes and mass formation using a newly developed injectable chitosan copolymer, and evaluate cartilage formation after injecting cell-thermosensitive copolymer complex in in vivo rabbit model. The MSCs were cultured in the chitosan-NIPAAm in vitro. Fluorescence- activated cell sort (FACS) analysis, viability test, collagen type I,II,X formation andthe aggrecan levels were examined. These cultured cells can be easily recovered from a copolymer gel by simply lowering the temperature. An animal study was performed to assess cartilage formation in the submucosal layer of the bladder of rabbits. The cartilage formation could be detected. This can be used to treat vesicoureteral reflux, reflux esophagitis or urinary incontinence by the effective tissue bulking effect. This is a simple method (sol-gel technique in LCST), and good cartilage formation occurs in the bladder tissue. |
| 저자 | 지경수1, 박형달1, 한상원2, 이진우2, 박기동1 |
| 소속 | 1아주대, 2연세대 |
| 키워드 | Chitosan; Thermosensitive polymer; urinary application |
