| 초록 |
Cell adhesion can be divided into three grades of adhesiveness: (1) early adhesiveness, meaning that the cell is attached but not spread (2) intermidiate adhesiveness characterized by a spread cell that lacks stress fibers and focal adhesions, and (3) late adhesiveness indicationg a spread cell with stress fibers and focal adhesions. Early adhesiveness is important because it is the first step to attach on polymers, and cell attachment results in cell growth, differentiation, viability, spreading. Cell attachment can be controlled by biological, chemical and mechanical properties. Cell adhesion could be controlled according to the properties chemical composition, hydrophobicity/hydrophilicity and mechanical parameter such as roughness. So we examined that what mechanism plays a predominat role of chondrogenic cell [mesenchymal stem cells (MSCs) and chondrocytes] adhesion under serum free conditions to attach on biodegradable polymer surfaces, respectively. Biodegradable polymers of PLGA(Poly lactic-co-glicolic acid = 75:25), PLA(Poly lactide), PCL(Poly-ε-carprolacton) were evaluated. Also to evaluate the biological reactions involved in cell - polymer interactions, integrin β1, one of the cell adhesion molecules, was blocked with monoclonal antibody. Heparin and L-arginine were also used in the cell attachment testing of new materials for cartilage tissue engineering. The possible mechanisms of cell adhesion on biodegradble polymeric surfaces will be discussed. |
