| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.98 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | The effect of mechanical stimulation of tissue engineered articular cartilage |
| 초록 | Introduction It is known that complex loading is concerned in the development and maintenance of articular cartilage in the body1. It means the compressive mechanical stimulation is a very important factor for formation of articular cartilage using a tissue engineering technique2. The objective of this study is to engineer cartilaginous constructs on a mechano-active bioreactor and to evaluate the effect of dynamic compression for regeneration of cartilage. Materials and Methods Very elastic poly(lactide-co-carprolactone)(5:5) copolymer was synthesized in the presence of stannous octoate. The scaffolds with 85 % porosity and 300~500 μm pore size were fabricated by a gel-pressing method. Cell-polymer constructs were cultivated on a dynamic compressive bioreactor in vitro. Also, the chondrocytes-seeded constructs cultured at static or dynamic condition for 10 days were further implanted at the backs of nude mice subcutaneously. Cell-polymer constructs were characterized by SEM, MTS assay, Glycosaminoglycans (GAGs) and collagen quantitative analysis, and histological studies. Results and Discussion The collagen and GAG assays showed that chondrogenic differentiation was sustained and enhanced significantly and chondrial extracellular matrix was increased through mechanical stimulation. The in vitro results were agreed well with the in vivo studies to confirm the effect of dynamic compressive stimulation over the static condition. Although the water and collagen contents of implants had no difference significantly, GAGs contents of implants stimulated by compressive foces were higher than them without stimulation. Histological analysis of the specimen retrieved at 8 weeks using Hematoxylin and eosin (H&E) staining showed that implants stimulated mechanically by compression formed mature and well-developed cartilaginous tissue, as evidenced by chondrocytes within lacunae. Safranin O and Alcian blue staining indicated an abundant accumulation of GAGs (Fig. 1). Fig. 1. Histological studies of implants 8 weeks after implantation Acknowledgements This work was supported by Korea Ministry of Science and Technology, M6-0302-00-0017. References 1. O. Demarteau, et. al., Biochem Biophy Res Comm 2003;310:580-588 2. 3. Eric M. Darling, et. Al., Annu Biomed Eng 2000;2:1114-1124 |
| 저자 | 정영미1, 유희진1, 김상헌1, 김수현1, 김영하1, Jun Xie2, Takehisa Matsuda3 |
| 소속 | 1한국과학기술(연), 2Department of Biomedical Engineering, 3Kyushu Univ. |
| 키워드 | bioreactor; tissue engineered cartilage |
