| 초록 |
In this study, we synthesized cationic dendrimer conjugated with L-arginine and γ-aminobutyric acid (GABA) as a nonviral gene delivery vector. GABA was selected as a spacer molecule to provide hydrophobicity and flexibility. PAMAM G4-GABA-Arg and PAMAM G4-Arg-GABA were synthesized and characterized. Complex formation with pDNA was confirmed by agarose gel retardation assay. Transfection efficacy and cytotoxicity of synthesized PAMAM derivatives was evaluated by luciferase expression assay and WST-1 assay, respectively. PAMAM G4-GABA-Arg showed enhanced transfection efficiency compared to PAMAM G4-Arg-GABA. Also, cytotoxicity of PAMAM G4-GABA-Arg showed negligible cytoxicity. |
