| 초록 |
Detection of heavy metal ions in aqueous system including human blood plasma is an important issue because of their adverse effects on human health and the environments. Among them, Pb2+, and Hg2+are the major elements requiring a critical caution because of the severe health effects. While various detection methods have been developed, the heavy metal specific oligonucleotides such as metal dependent DNAzyme and Hg2+ aptamers have improved the detection sensitivity and specificity significantly. However, further improvement for the detecting sensitivity and feasibility are still open. Specially, no scheme is reported to detect Pb2+ and Hg2+ simultaneously. For the dual detection, a fluorescence signal change is more suitable rather than color change in the presence of the metal ions.Therefore, we devised a scheme to generate fluorescence signal combining the DNAzyme and aptamer on gold nanoparticles which act as a super quencher for two different fluorophores . In the presence of the metal ions, the fluorescence signal can be turned on because of the enzymatic cleavages of the substrate by DNAzymes for Pb2+ and the conformational change for Hg2+ whereas the nanoparticles are in latent in the absence of the metal ions. |
