| 초록 |
Three-dimensional (3-D) culture dish with ordered honeycomb pattern as an artificial human ovary where cells can behave as they do in vivo demonstrates a great application potentials in biotechnology. Here, we report on a very simple, low-cost, scalable and single-step method to directly create ordered controllable honeycomb patterns on polymeric substrates including the commercial Petri dish in normal air. The method based on the combination of the methanol accumulation-induced phase separation and the dip-coating technique in which a mixture of chloroform and methanol is exploited not only to induce a ternary solution but also to guarantee the formation of highly ordered honeycomb structure on the substrate. The surface topology of honeycomb substrate is effectively controlled by varying experimental conditions and the obtained honeycomb structure is indeed a part of the substrate revealing an increase in the structure’s stability. Moreover, honeycomb-structured Petri dishes with controllable pore arrays produced using this method shows good cell adhesion and proliferation as an evidence for use in biomedical applications. |
